A375 Transfection Reagent (Melanoma Cells, CRL-1619)

A375 cells have been used as test organisms that also produce paracrine factors for prolonged culture of mesenchymal stromal cells (MSCs). Melanoma is the most lethal formal of skin cancer and is responsible for more than 75% of skin cancer-related deaths. The A375 cell line is relevant to melanoma studies, and it was originally obtained from a 54-year-old female. A375 cells are epithelial-like with adherent properties, hypotriploid, and have a modal number of 62 chromosomes. The cells produce large amounts of amelanotic melanomas in xenografts of NIH swiss mice treated with anti-thymocyte serum. A375 cells are suitable for transfection applications, especially those that deal with oncogenetic factors that are tied to melanomas. For the A375 transfection reagent , visit the Altogen Biosystems website.

Altogen Biosystems is a ­current global provider of transfection reagents and contract research services for researchers in gene-related fields. Some of the services that are offered include assay development, fast generation of stably-transfected cell lines, and other laboratory services including siRNA screening. For an A375 cell xenograft model  and other valuable resources visit the Altogen Biosystems website.

The A375 cancer cell line (CRL-1619) was established using the skin cells from female patient that was diagnosed with melanoma, A375 cell line is used as a transfection host for melanoma research.  Tranfection reagents designed for A375 cells are commonly used for melanoma research studies by cancer research centers worldwide. Transfection reagents for melanoma cell lines have been developed and manufactured by companies such as  Altogen Biosystems . These transfection reagents can be used in research studies involving the co-transfection of RNA and DNA molecules, such as plasmid DNA and small interfering RNA (siRNA).  Transfection Reagent for A375 Cell s

A-431 (CRL-1555) cancer cell line was established by DJ Giard and SA Aaronson from the solid tumor of a female patient suffering from epidermoid carcinoma; in cancer research, A431 cell line is used in medical research to study oncology treatments of skin cancer and is commonly used as a transfection host cell line.  A431 transfection reagent is manufactured by  Altogen Biosystems  and  has been reported to have a 81% transfection efficacy for the delivery of small RNA molecules. This reagent is functionally tested in vitro and has been proven to facilitate the development of A431 stable cell lines. In addition, A-431 transfection reagents for epidermoid carcinoma cell line work in the presence of serum and it is used in both reverse and standard transfection protocols.  Transfection Reagent for A431 Cells