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A431 Transfection

A-431 (CRL-1555) cancer cell line was established by DJ Giard and SA Aaronson from the solid tumor of a female patient suffering from epidermoid carcinoma; in cancer research, A431 cell line is used in medical research to study oncology treatments of skin cancer and is commonly used as a transfection host cell line. 

A431 transfection reagent is manufactured by Altogen Biosystems and has been reported to have a 81% transfection efficacy for the delivery of small RNA molecules. This reagent is functionally tested in vitro and has been proven to facilitate the development of A431 stable cell lines. In addition, A-431 transfection reagents for epidermoid carcinoma cell line work in the presence of serum and it is used in both reverse and standard transfection protocols. 

Transfection Reagent for A431 Cells

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A375 Cell Line

A375 is a tumorigenic cell line that was taken from skin cells of a 54-year-old woman who was diagnosed with malignant melanoma. A375 cells are hypotriploid and have a chromosomal modal number of 62. They display an epithelial morphology, as well as grow in adherent monolayers when cultured  in vitro . The A375 cell line can be used for either in vivo or in vitro  transfection, and Altogen Biosystems offers both an A375 xenograft mouse model and an A375 Transfection Reagent . The A375 cell line is useful for studying skin cancer, as well as other skin infections.

Bufotalin Induces A375 Cell Cycle Arrest and Apoptosis

Bufotalin, an active component of Venenum bufonis, has long been used as an anti-tumor drug in China and has proven anti-tumor effects in cancer types. In the following study, researchers use MTT and colony formation assays to analyze the effects of bufotalin on A375 melanoma cells. Results show that bufotalin induces A375 cell cycle arrest at the G2/M phase by up-regulating and down-regulating specific protein expressions. Furthermore, bufotalin up-regulates apoptosis-related proteins, in turn inducing A375 cell apoptosis. These results indicate that bufotalin may prove effective in melanoma treatment. [ LINK ] An  A375 Transfection Reagent  is commercially available from  Altogen Biosystems , which includes standard and reverse protocols to transfect A375 cells. A xenograft model of this cell line can be found  here .

About the A375 Cell Line

A375 cells have been used as test organisms that also produce paracrine factors for prolonged culture of mesenchymal stromal cells (MSCs). Melanoma is the most lethal formal of skin cancer and is responsible for more than 75% of skin cancer-related deaths. The A375 cell line is relevant to melanoma studies, and it was originally obtained from a 54-year-old female. A375 cells are epithelial-like with adherent properties, hypotriploid, and have a modal number of 62 chromosomes. The cells produce large amounts of amelanotic melanomas in xenografts of NIH swiss mice treated with anti-thymocyte serum. A375 cells are suitable for transfection applications, especially those that deal with oncogenetic factors that are tied to melanomas. For the A375 transfection reagent , visit the Altogen Biosystems website.