A375 is a tumorigenic cell line that was taken from skin cells of a 54-year-old woman who was diagnosed with malignant melanoma. A375 cells are hypotriploid and have a chromosomal modal number of 62. They display an epithelial morphology, as well as grow in adherent monolayers when cultured in vitro . The A375 cell line can be used for either in vivo or in vitro transfection, and Altogen Biosystems offers both an A375 xenograft mouse model and an A375 Transfection Reagent . The A375 cell line is useful for studying skin cancer, as well as other skin infections.
In this study, researchers test the anti-tumor effects of combined IL-24/OSM adenoviral-mediated tumor suppressor gene co-transfer in A375 cells. Interleukin-24 (IL-24) is a cytokine-tumor suppressor that exhibits anti-tumor properties. Oncostatin M (OSM) inhibits the proliferation of various solid tumor cell lines. Researchers combined IL-24 and OSM to form a bicistronic adenovirus and tested its effect on A375 cells in vivo and in vitro . Results indicate that IL-24/OSM reduces certain protein expressions in A375 cell cycles and this activity is closely associated with the activation of an apoptotic pathway and inhibition of tumor angiogenesis. Therefore, combining two or more tumor suppressors may lead to a new strategy of treating melanoma. [ LINK ] An A375 Transfection Reagent is commercially available from Altogen Biosystems , which includes standard and reverse protocols to transfect A375 cells. A xenograft model of this cell line can be found here ...