A375 Transfection Reagent (Melanoma Cells, CRL-1619)

In this study, researchers test the anti-tumor effects of combined IL-24/OSM adenoviral-mediated tumor suppressor gene co-transfer in A375 cells. Interleukin-24 (IL-24) is a cytokine-tumor suppressor that exhibits anti-tumor properties. Oncostatin M (OSM) inhibits the proliferation of various solid tumor cell lines. Researchers combined IL-24 and OSM to form a bicistronic adenovirus and tested its effect on A375 cells in vivo and in vitro . Results indicate that IL-24/OSM reduces certain protein expressions in A375 cell cycles and this activity is closely associated with the activation of an apoptotic pathway and inhibition of tumor angiogenesis. Therefore, combining two or more tumor suppressors may lead to a new strategy of treating melanoma. [ LINK ] An  A375 Transfection Reagent  is commercially available from  Altogen Biosystems , which includes standard and reverse protocols to transfect A375 cells. A xenograft model of this cell line can be found  here ...

Bufotalin, an active component of Venenum bufonis, has long been used as an anti-tumor drug in China and has proven anti-tumor effects in cancer types. In the following study, researchers use MTT and colony formation assays to analyze the effects of bufotalin on A375 melanoma cells. Results show that bufotalin induces A375 cell cycle arrest at the G2/M phase by up-regulating and down-regulating specific protein expressions. Furthermore, bufotalin up-regulates apoptosis-related proteins, in turn inducing A375 cell apoptosis. These results indicate that bufotalin may prove effective in melanoma treatment. [ LINK ] An  A375 Transfection Reagent  is commercially available from  Altogen Biosystems , which includes standard and reverse protocols to transfect A375 cells. A xenograft model of this cell line can be found  here .

In the following study, researchers test the effect of microRNA-183 (miR-183) on A375 melanoma cells. Lentiviral transfection was used to construct an A375 cell line with stable miR-183 over-expression or knockdown. Additionally, r everse transcription-quantitative polymerase chain reaction was used to measure the change of miR-183 expression in A375 cells, and  a scratch and Transwell assay was used to determine its effects on A375 cell migration. Results show that miR-183 expression inhibits A375 migration and invasiveness. This effect is supported by the fact that  Ezrin and MMP-9, two known promoters of melanoma metastasis, are revealed to be negatively regulated by miR-183. Therefore, miR-183 may inhibit A375 melanoma cell migration by targeting and inhibiting Ezrin and MMP-9 expression. This study may ultimately contribute novel insights to the development of anti-metastasis gene therapy. [ LINK ] An  A375 Transfection Reagent  is commercially available f...

Sutherlandia frutescens is a plant native to Southern Africa that has been traditionally used for treating various ailments and even reported to have anticancer activity. Based on claims of anticancer activity, the plant has been locally dubbed the "cancer bush".  Sutherlandia frutescens is commercially available in the form of tablets or tinctures and consuming the product is not associated with any toxic side effects. In the following study, scientists research the effect of  Sutherlandia frutescens on A375 melanoma cells. The aim of the study is to find evidence of whether or not the plant extract induces apoptosis on the A375 melanoma cell line. Results show that  Sutherlandia frutescens is cytotoxic in a dose- and time-dependent manner and induces caspase-dependent and caspase-independent apoptosis in A375 cells. Gene analysis conducted during the study also molecular targets the  Sutherlandia frutescens extract, which include:  angiogenesis, metastasis, EM...